Indian Journal of Human Genetics
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ORIGINAL COMMUNICATION
Year : 2005  |  Volume : 11  |  Issue : 1  |  Page : 31-34

Heterozygosity and allele frequencies of the two VNTRs (ApoB and D1S80) in Iranian population


1 Akbari Medical Genetic Laboratory, Genetic Research Center, University of Welfare and Rehabilitation Sciences, Tehran, Iran
2 Akbari Medical Genetic Laboratory, University of Welfare and Rehabilitation Sciences, Tehran, Iran

Correspondence Address:
Nejat Mahdieh
Genetics Research Center, University of Welfare and Rehabilitation Sciences, Tehran
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0971-6866.16292

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Genetic markers are used for identity testing and paternity analysis depends on knowing the allele frequencies in the population. Minisatellites show allelic variability in the number of repeat units. We have studied the allele frequencies and heterozygosity of two VNTRs (ApoB and D1S80) in Iranian populations. A total of 96 and 82 chromosomes were analyzed by PCR and gel electrophoresis for ApoB and D1S80 respectively. In the ApoB system, allele 37 was the most common followed by allele 35 whereas allele 23 was the most common followed by allele18 at the D1S80 locus. Observed heterozygosity was relatively low in ApoB than D1S80 locus, however, no significant differences were found between observed and expected heterozygosity.


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