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2003| January-June | Volume 9 | Issue 1
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Serum Adenosine deaminase activity and C-reactive protein levels in unstable angina
Surekha H Rani, Dayasagar V Rao, Shiva Prakash M, Jyothy A
January-June 2003, 9(1):17-20
In unstable angina (USA) patients, immunological responses contributing to inflammation play a vital role in plaque rupture and thrombosis causing stroke. In the present study an attempt is made to estimate the levels of adenosine deaminase activity, an immunoenzyme marker and C-reactive protein, a marker of inflammation in USA patients. 45 patients presenting USA and 50 age and sex matched healthy controls were included in the study. Serum ADA activity was measured spectrophotometrically at 630nm and serum C-reactive protein was detected using Avitex CRP kit, which is a rapid latex agglutination test. The Mean ADA levels were 41.15 ± 11.04 in patients and 20.71±5.63 in controls and 66.6% of patients and none of the controls were positive to CRP. The present study observed the importance of ADA as a serum marker in addition to CRP for assessing the immune response in USA patients.
Alternate strategies for carrier detection and antenatal diagnosis in haemophilias in developing countries
Shrimati Shetty, Kanjaksha Ghosh, Dipika Mohanty
January-June 2003, 9(1):5-9
Carrier detection and prenatal diagnosis constitute an important component of haemophilia management . Recent advances in molecular biology allows us to use the tools of molecular biology to give such a diagnosis early in the pregnancy with a much higher confidence. Because of lyonisation, diagnosis of a carrier by factor assay is imperfect and hence lacks sensitivity. Molecular diagnosis in such cases is robust.There are several techniques by which this diagnosis can be made.Though the preferred method is to do direct mutation studies, yet the complexities of factor VIII and factor IX genes may not make this approach easy or cost effective. Hence depending on the capability of the laboratory, education status of the family, availability of data through several generations and economic situation of the country, a combination of these techniques need to be adopted for optimum results. These techniques are broadly classified as indirect techniques through linkage analysis or direct detection of affected genes by a combination of screening and sequencing techniques. Occasionally in our country even all the gene based techniques may prove inadequate and we may have to give prenatal diagnosis by antigen and clotting activity assay of the defective factor by cordocentesis between 17-20 weeks of gestation. For any prenatal diagnosis of haemophilia, prior detection of fetal sex either by USG or by molecular technique is necessary to decide whether any further work up is necessary or not? The present article describes various algorithms of carrier detection prenatal diagnosis of haemophilia that was found suitable in our country.
Radiation-induced chromosomal hot spots at G
stages of human lymphocytes in culture
January-June 2003, 9(1):21-24
Radiation-induced chromosomal break points in cultured lymphocytes of normal healthy individuals as well as of those with certain genetic disorders are reported to be localized at certain specific loci (hot spots). These reports are based on studies carried out in lymphocytes irradiated at G
stage. The present study examines whether the location of hot spots and the frequency seen in cells irradiated at G
are similar to those irradiated at G
stage of the cell cycle and also tests whether cells of patients exhibit hot spots on irradiation.The results showed that the radiation induced chromosomal break points to be similar in those irradiated are G
stages of the cell cycle and also that cells of patients exhibited chromosomal hot spots.
Pairwise MtDNA-HVRII sequence differences and geographic maternal distances among Korku, an Austro-Asiatic tribe in Central India
Raghavendra V Rao, Kumarasamy Thangaraj, Alla G Reddy, V Sridhar, Lalji Singh
January-June 2003, 9(1):25-28
Ethno-linguistic and recent molecular evidences were equivocal that tribes belonging to Austro-Asiatic linguistic group were the earliest 'out of Africa' migrants in the Indian sub-continent dating back to approximately 60,000 YBP. They comprise of few endogamous groups and were settled in eastern and central India. In the present study it is found among Korku, an endogamous Austro-Asiatic tribe settled in central India, that oldest females pairwise, maternal geographical distances were inversely related with mtDNA-HVRll control region sequence differences, mismatch distribution was unimodal and the most common haplotype had wide geographical distribution. The empirical findings lead to 1) the possibility of an explanation that the Korku, though showed demographic expansion, the place of expansion may not be the area of their present habitation 2) the scope of combining information on maternal distances and mtDNA sequence diversity in deriving demographic history of populations.
HLA A19 subtypes and B loci related haplotype in selected caste groups from the Indian population
January-June 2003, 9(1):13-16
The Indian population has been broadly classified as Aryans of Northern India and Dravidians of South India. The present study was undertaken to compile available data and investigate the genetic diversity of HLA A19 subtypes in Indians and its associated B locus haplotype frequency distribution at the population level. The study revealed that A33 was common among the selected North Indian caste groups (Aryans) while A31 was common among the selected South Indian caste groups (Dravidians). The haplotypes A33-B44 and A19-B35 were characteristic to Aryans while haplotypes A19-B22 and A19-B7 were characteristic to Dravidians. Further novel haplotypes such as A19-B14 and A33-B49 were unique to Parsis and Sourastran caste. A low frequency of A29 was observed among the A19 subtype repertoire. Prevalence of HLA A33 and A31 among North Indians (Aryans) and South Indians (Dravidians) along with their unique haplotypes may be a consequence of the founder effect, racial admixture or selection pressure due to environmental factors among this population.
Chromosomal Instability in peripheral blood lymphocytes in patients with malignancy
January-June 2003, 9(1):10-12
There are lot of studies on the cytogenetic changes in solid tumours but there are very few studies on the cytogenetic changes in peripheral blood by mphosytes in patients with malignancy. In the present study we evaluated peripheral blood lymphocyte cytogenetic changes on short term culture. Twenty four out of 250 patients with various malignancies showed some aberration of karyotypes in peripheral lymphocytes (9-6%) suggesting an underlying genetic instability in these cancer patients or alternatively demonstrating that these changes could be related to exposure to environmental mutagens.
Is prenatal diagnosis the solution to control some of the genetic disorders in developing countries? YES
January-June 2003, 9(1):3-4
Human herpesvirus 6: Catalyst of genomic lesion in dysfunctional hematopoiesis ?
January-June 2003, 9(1):29-30
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